Rapid, high-throughput assessment of protein stability on the Rotor-Gene Q cycler

The assessment and optimization of protein stability is crucial for many diverse applications. Determination of the melting temperature of a protein provides a straightforward method of protein stability measurement. Such measurements can be easily performed using the Rotor-Gene Q cycler with up to 100 samples in parallel. The Rotor-Gene Q cycler provides unmatched temperature uniformity due to its unique centrifugal rotary design and single short optical pathway. Its detection precision is an important advantage for techniques such as real-time PCR or protein stability measurement. In this article, we describe how to set up and perform protein stability measurement using a Rotor-Gene Q cycler. We also present application data demonstrating the potential of this technique for drug discovery.